Also shown is a silver-stained comet example in which the percentage of DNA contained in the tail is calculated.
Also, illumination correction is used to reduce background fluorescence prior to measurement. Here, the measurement of interest is the length and intensity of the comet tail. This is a simple example of a DNA damage assay using single cell gel electrophoresis.
#Cellprofiler intensity measurements how to#
This pipeline demonstrates how to identify these clumpy cells and obtain morphological, intensity and texture measurements.Ī simple pipeline that identifies and counts tumors in a mouse lung, and then measures their size. In contrast to the HT29 cells, Drosophila Kc167 cells are a highly textured and clumpy cell type. This pipeline demonstrates how to accurately identify these cells and how to measurements cellular parameters such as morphology, count, intensity and texture. Human HT29 cells are fairly smooth and elliptical. These pipelines are made for simple cellular and tissue image assays, and include some basic measurements.
OLD CellProfiler example images and pipelines (Prior to 4.0) These examples are outdated, please see our current Examples page for the updated versions.
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